Composite
Part:BBa_J56004:Design
Designed by: Matt Eames Group: iGEM2006_UCSF (2006-10-31)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 181
Illegal PstI site found at 1312 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1464
Illegal PstI site found at 181
Illegal PstI site found at 1312 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 181
Illegal PstI site found at 1312 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 181
Illegal PstI site found at 1312
Illegal NgoMIV site found at 1613
Illegal AgeI site found at 1290 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon-optimized as to avoid recombination with highly similar PheTar
Source
Synthesized by DNA 2.0