Composite

Part:BBa_J56004:Design

Designed by: Matt Eames   Group: iGEM2006_UCSF   (2006-10-31)


Tar EA400 binds to BBa_J56001


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 181
    Illegal PstI site found at 1312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1464
    Illegal PstI site found at 181
    Illegal PstI site found at 1312
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 181
    Illegal PstI site found at 1312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 181
    Illegal PstI site found at 1312
    Illegal NgoMIV site found at 1613
    Illegal AgeI site found at 1290
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon-optimized as to avoid recombination with highly similar PheTar


Source

Synthesized by DNA 2.0

References